Greetings, DD'ers and Happy Sunday! It's been a fairly brutal spring and summer as far as work goes, so I have not been able to scrap much. Heck, I have not been able to eat or sleep much! However, as the sale of Patti's products heads into its final days, I wanted to take time to participate in the Honoring Pattie challenge. One of her templates that I recently purchased was perfect for the story of this year's 4th of July. Besides space for several photos, there was plenty of space for me to tell one of my long-winded stories and actually get it on the same page as the photos! Smile


Oh, and I feel compelled to explain to those who may not know that the word "pooped" is slang for tired, although some of you might use this word in a completely different context. Smile See, I was going for a kind of speech cadence here: "Red, White and Blue" becomes "Red, White and Pooped." Get it? <sound of crickets chirping> Okay, just me then. Aaaaaaanway, my niece took one look at the title and said, "Ewwww!" so I thought I'd better explain myself! Smile


This page tells the story of why Mr. Squeak and I were not able to have a more traditional 4th of July celebration this year, and it also tells the story of why I've been so very busy. I use a bit of work place jargon in the telling, so feel free to ignore those bits. All you really need to know is that I was tasked with getting something done in 2-3 months, something that took me 2 years to do originally, and I am sooo pooped! Smile


Okay, back to the salt mines. See you in another couple of months! Sigh.


Journaling reads:
"Ah, the 4th of July, a day of celebration, a day that marks the birth of our country, a day filled with cook-outs, picnics, baseball games, fireworks, music and family get-togethers. A day filled with splitting HEK cell plates, virus banding, ligations, transformations and maxi preps. Wait! Wha...?!?!?


The story is this...


It all started back in April. xxxx yyyy, a graduated student in the lab, reported that the anti-GFP antibody we use to attach GFP-myosin to the beads in the trap was causing the trap stage to stick, and that this sticking was particularly noticeable during oscillating stage measurements, making it difficult to separate signal from noise.


After a brief discussion it was decided that all of our cardiac myosin constructs should use a different attachment system, one that used the so-called affinity clamp: a C-term peptide that binds to a directed-evolution PZD domain, and that these constucts needed either a 1x or a 2x GSG linker.


It fell to me to design and construct the plasmids that would make all of this happen. When I made all of the GFP-tagged plasmids that we are currently using to make cardiac myosin protein, I took a lesiurely two years, making plasmids only as we needed them. Now I have to make all of those same plasmids only with three different attachment systems and make them all in about two months. Ugh!


Since the end of April I have been working 12-hr days, 7 days a week. The spring and summer have truly sucked. I can’t remember the last time I was this exhausted.


I was really, really looking forward to July 4th this year; since it fell on a Thursday I had plans to make it a 4-day weekend and go someplace--ANY place!!!--with my best beloved for a bit of RnR. Then I learned that both xxxx xxx and yyyy yyyyy would be gone that week, and someone needed to stay in town and take care of the cells. GUESS who that someone was? Sigh.


After whining about it for a while, I put on my big girl panties and dealt with it. I bought some festive lights and a table decoration at the local Dollar Store and decorated my lab bench. I decided I would do only what absolutely, positively needed doing that day and not one thing more so that I could get out of the lab before dark. Rather than rush home and cook, I opted to pick up some barbecue from Armadillo Willy’s. Instead of fighting traffic to get to the local fireworks display, we walked out to the Central Expressway overpass and watched the display from there. After, we walked home and I fell into bed, asleep almost immediately. Red, white and blue? More like red, white and pooped!"


Credits
Cassie Jones
Laying It All Out No. 03: Custom Stickers and Clever Layering
How’d They Do That? No. 13: Bending Shadows


Pattie Knox
Amuse Me Alphabet: Red
Its Black and White: Felt Alpha Collection
Hoppy Birthday Page Kit (alpha)
Shimmer Me Tidbits: Fireworks
Speed Byte No. 070
Yankee Doodle Paper Pack


Thanks for stopping by!